In this manuscript, we discuss the benefits and drawbacks of different 3D microfluidic devices. To further improve or maintain hepatic functions, 3D cultivation has been combined with perfusion. As it has been demonstrated that hepatocytes embedded between two sheets of collagen maintain their function, various hydrogels and scaffolds for the 3D cultivation of hepatocytes have been developed. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions result in a loss of hepatocyte morphology and of liver-specific functions, such as drug-metabolizing enzymes and transporters. One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |